Variations in serum concentrations of C-reactive protein, ceruloplasmin, lactoferrin and myeloperoxidase and their interactions during normal human pregnancy and postpartum period

Ewa Skarżyńska; Hanna Zborowska; Artur J Jakimiuk; Maria Karlińska; Barbara Lisowska-Myjak

doi:10.1016/j.jtemb.2017.11.015

Variations in serum concentrations of C-reactive protein, ceruloplasmin, lactoferrin and myeloperoxidase and their interactions during normal human pregnancy and postpartum period

J Trace Elem Med Biol. 2018 Mar:46:83-87. doi: 10.1016/j.jtemb.2017.11.015. Epub 2017 Nov 29.

Authors

Ewa Skarżyńska¹, Hanna Zborowska², Artur J Jakimiuk³, Maria Karlińska⁴, Barbara Lisowska-Myjak⁵

Affiliations

¹ Department of Biochemistry and Clinical Chemistry, Medical University of Warsaw, Warsaw, Poland. Electronic address: ewaskarzynska@wp.pl.
² Department of Laboratory Diagnostics, Medical University of Warsaw, Warsaw, Poland.
³ Institute of Mother and Child Reproductive Health Department, Warsaw, Poland.
⁴ Department of Medical Informatics and Telemedicine, Medical University of Warsaw, Warsaw, Poland.
⁵ Department of Biochemistry and Clinical Chemistry, Medical University of Warsaw, Warsaw, Poland.

PMID: 29413114
DOI: 10.1016/j.jtemb.2017.11.015

Abstract

Background: Serum proteins may provide information about homeostasis of redox status and inflammatory processes also during pregnancy. The aim of the study was to assess the dynamics of changes in serum concentrations of C-reactive protein (CRP), ceruloplasmin (CP), lactoferrin (LF) and myeloperoxidase (MPO) and their interactions during normal pregnancy and the postpartum period.

Methods: The concentrations of proteins were measured in serum (n=113) from pregnant in consecutive trimesters and in postpartum period (n=28) and in non-pregnant women (n=17), using immunoturbidimetric assays (CRP, CP) and ELISA Kits (LF, MPO).

Results: The concentrations [mg/dl] CP and CRP (mean±SD respectively): second trimester (43.1±6.2; 0.49±0.57), third trimester (44.5±5.8; 0.41±0.37), postpartum (42.39±6.4; 4.15±3.6) were higher than in the first trimester (33.0.5±8.7; 0.31±0.36) or non-pregnant women (24.12±7.4; 0.12±0.13). The increases in concentrations of CP and CRP between the first and the second trimesters were by approximately 35% and 50% respectively and the correlation coefficients in the first trimester and in non-pregnant women were twice higher than in the second trimester and the postpartum period. The concentrations [μg/ml] LF and MPO were no significant differences (mean±SD respectively): first (6.19±4.54; 0.17±0.12), second (5.68±4.4; 0.14±0.08), third (6.34±6.98; 0.17±0.14), the postpartum (4.86±3.64; 0.25±0.4), and non-pregnant (3.9±2.56; 3.2; 0.14±0.05). However, significant correlations were established (p<0.05) between MPO and LF in all groups and between the following ratios CRP/LF vs CP/MPO and CRP/MPO vs CP/LF.

Conclusions: The concentrations of proteins synthesized by the liver (CP, CRP) dynamically increase during consecutive trimesters of pregnancy unlike neutrophil-derived proteins (LF, MPO). Statistically significant correlations between the proportions of the serum proteins may suggest their combined role for the maintenance of homeostasis during pregnancy.

Keywords: C-reactive protein; Ceruloplasmin; Lactoferrin; Myeloperoxidase; Pregnancy; Serum.

MeSH terms

Adolescent
Adult
C-Reactive Protein / metabolism*
Ceruloplasmin / metabolism*
Female
Homeostasis
Humans
Lactoferrin / metabolism*
Oxidation-Reduction
Peroxidase / metabolism*
Postpartum Period / metabolism*
Pregnancy / metabolism*
Pregnancy Trimesters
Protein Binding
Young Adult

Substances

C-Reactive Protein
Peroxidase
Ceruloplasmin
Lactoferrin