A high throughput methodology for susceptibility testing of Mycobacterium tuberculosis isolates

J Microbiol Methods. 2018 Mar:146:64-67. doi: 10.1016/j.mimet.2018.02.001. Epub 2018 Feb 2.

Abstract

MICs for eleven anti-TB drugs with M. tuberculosis isolates were obtained by means of agar dilution with multi-point inoculation. The results were compared with classic agar dilution and the MTT assay. The multi-point inoculation method was reproducible with all drugs and correlated with classic agar dilution and MTT assay. This methodology can be used for routine breakpoint drug susceptibility testing (DST) and for MIC determination.

Keywords: Agar-dilution; Drug susceptibility test; MIC; Multi-point inoculation; Mycobacterium tuberculosis.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Antitubercular Agents / pharmacology*
  • Bacteriological Techniques / instrumentation
  • Bacteriological Techniques / methods
  • Culture Media
  • Diagnostic Tests, Routine / instrumentation
  • Diagnostic Tests, Routine / methods*
  • High-Throughput Screening Assays / instrumentation
  • High-Throughput Screening Assays / methods*
  • Humans
  • Microbial Sensitivity Tests / instrumentation
  • Microbial Sensitivity Tests / methods*
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / isolation & purification
  • Reproducibility of Results
  • Tuberculosis, Multidrug-Resistant / diagnosis
  • Tuberculosis, Multidrug-Resistant / microbiology

Substances

  • Anti-Bacterial Agents
  • Antitubercular Agents
  • Culture Media