Quack: A quality assurance tool for high throughput sequence data

Adam Thrash; Mark Arick 2nd; Daniel G Peterson

doi:10.1016/j.ab.2018.01.028

Quack: A quality assurance tool for high throughput sequence data

Anal Biochem. 2018 May 1:548:38-43. doi: 10.1016/j.ab.2018.01.028. Epub 2018 Feb 1.

Authors

Adam Thrash¹, Mark Arick 2nd¹, Daniel G Peterson²

Affiliations

¹ Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA.
² Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA. Electronic address: dp127@msstate.edu.

PMID: 29410015
DOI: 10.1016/j.ab.2018.01.028

Abstract

The quality of data generated by high-throughput DNA sequencing tools must be rapidly assessed in order to determine how useful the data may be in making biological discoveries; higher quality data leads to more confident results and conclusions. Due to the ever-increasing size of data sets and the importance of rapid quality assessment, tools that analyze sequencing data should quickly produce easily interpretable graphics. Quack addresses these issues by generating information-dense visualizations from FASTQ files at a speed far surpassing other publicly available quality assurance tools in a manner independent of sequencing technology.

Keywords: DNA; FASTQ; Quality assurance; Quality check; Visualization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

High-Throughput Nucleotide Sequencing / methods*
Sequence Analysis, DNA / methods*
Software*