Classic and conventional procedures in molecular cloning are inherent compositions in modern molecular biological experiments and are frequently involved in daily laboratory activities. They take up the majority of the total time input in spite of the availability of well-designed specialized commercial kits. A similar situation is also in the field of biotechnology. Fortunately, microwave/ultrasonic irradiation has been found to be capable of speeding up these processes, such as proteolysis in sample preparation for proteomics research, and digestion, ligation, (de)phosphorylation of DNA with the corresponding enzymes, even the introduction of DNA samples to recipient cells, and biotransformation (e.g., the production of biodiesel). Microwave/ultrasonic irradiation, when used solely or in combination with other existing operations, makes it possible to finish these time-consuming processes in as short as 1 min with comparable or even improved efficiency, and there is no need of reagent upgradation. The adoption of irradiation is ideal because it eliminates any possible side effects of the chemicals used as performance enhancer(s) that will inevitably make the system more complicated at least. More notably, the needed irradiation in the laboratory can be generated by a common microwave oven or ultrasonic cleaner. Taken together, microwave/ultrasonic irradiation provides an accessible method to make the procedures mentioned above time- and cost- efficient. In this article, we reviewed the relevant literature and discussed the experiment and mechanism details.
Keywords: Biotransformation; Efficiency enhancement; Microwave irradiation; Molecular cloning; Sample preparation; Structure flexibility.