Among various methods now available to isolate distinct cell populations or even single cells for DNA/RNA and proteomic analysis, laser capture microdissection (LCM) offers a unique opportunity to study cells in their topological contexts. This chapter focuses on the preparation of LCM membrane slides, tissue staining and laser microdissection of cells of interest from frozen or formalin-fixed, paraffin-embedded glioblastoma tissue.
Keywords: Formalin-fixed, paraffin-embedded tissue; Frozen tissue; Gene expression microarray; Glioblastoma; Glioblastoma molecular profiling; Laser capture microdissection; Preanalytical conditions; Tumor cell enrichment; Tumor heterogeneity.