Adipose-derived stem cells decrease cardiomyocyte damage induced by porphyromonas gingivalis endotoxin through suppressing hypertrophy, apoptosis, fibrosis, and MAPK markers

Tung-Sheng Chen; Chia-Hua Kuo; Sarnai Battsengel; Lung-Fa Pan; Cecilia Hsuan Day; Chia-Yao Shen; Li-Chin Chung; V Vijaya Padma; Chen-Kai Yao; Yueh-Min Lin; Chih-Yang Huang

doi:10.1002/tox.22536

Adipose-derived stem cells decrease cardiomyocyte damage induced by porphyromonas gingivalis endotoxin through suppressing hypertrophy, apoptosis, fibrosis, and MAPK markers

Environ Toxicol. 2018 Apr;33(4):508-513. doi: 10.1002/tox.22536. Epub 2018 Jan 31.

Authors

Tung-Sheng Chen^{1

2}, Chia-Hua Kuo³, Sarnai Battsengel^{2

4}, Lung-Fa Pan^{5

6}, Cecilia Hsuan Day⁷, Chia-Yao Shen⁷, Li-Chin Chung⁸, V Vijaya Padma⁹, Chen-Kai Yao¹⁰, Yueh-Min Lin^{11

12}, Chih-Yang Huang^{2

13

14

15}

Affiliations

¹ School of Life Science, National Taiwan Normal University, Taipei, Taiwan.
² Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
³ Department of Sports Sciences, University of Taipei, Taipei, Taiwan.
⁴ Intermed Hospital, Chinggis Avenue 41, Khan-Uul district, 15th Khoroo, Uildver, Ulaanbaatar, 17040, Mongolia.
⁵ Division of Cardiology, Armed Force Taichung General Hospital, Taichung, Taiwan.
⁶ Department of Medical Imaging and Radiological Sciences of Central Taiwan, University of Science and Technology, Taichung, Taiwan.
⁷ Department of Nursing, MeiHo University, Pingtung, Taiwan.
⁸ Department of Hospital and Health Care Administration, Chia Nan University of Pharmacy and Science, Tainan County, Taiwan.
⁹ Department of Biotechnology, Bharathiar University, Coimbatore, 641 046, India.
¹⁰ Washington High School, Taichung, Taiwan.
¹¹ Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan.
¹² Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan.
¹³ Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan.
¹⁴ Faculty of Applied Sciences, Ton Duc Thang University, Tan Phong Ward, District 7, Ho Chi Minh City, 700000, Vietnam.
¹⁵ Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.

PMID: 29383833
DOI: 10.1002/tox.22536

Abstract

Heart failure is one of the complications related to periodontal disease. In addition to drugs or herbal medicines, stem cell therapy shows potential in the treatment of cardiomyopathy. This study investigates if stem cells exhibit beneficial effects on cardiomyocyte damage induced by porphyromonas gingivalis endotoxin (Pg-LPS). From the experimental results we find that Pg-LPS reduce cardiomyocyte viability via the activation of apoptosis, hypertrophy, fibrosis and MAPK signaling. Pg-LPS damaged cardiomyocytes co-cultured with adipose-derived stem cells (ADSC) increases cardiomyocyte viability through suppressing the pathological markers described above. Further evidence implies that survival marker, IGF1, secreted from ADSC, may play an important role in the Pg-LPS induced protective effect on cardiomyocyte damage.

Keywords: IGF1; cardiomyopathy; endotoxin; periodontal disease; stem cell.

MeSH terms

Adipose Tissue / cytology
Animals
Apoptosis
Cell Size
Cells, Cultured
Coculture Techniques
Endotoxins / metabolism*
Endotoxins / toxicity
Fibrosis
Humans
Lipopolysaccharides / pharmacology
MAP Kinase Signaling System
Myocytes, Cardiac / cytology*
Porphyromonas gingivalis / metabolism*
Rats
Stem Cells / cytology
Stem Cells / physiology*

Substances

Endotoxins
Lipopolysaccharides