Morpho-palynological study of Cyperaceae from wetlands of Azad Jammu and Kashmir using SEM and LM

Microsc Res Tech. 2018 May;81(5):458-468. doi: 10.1002/jemt.22999. Epub 2018 Jan 31.

Abstract

In this study 12 species of Cyperaceae have been studied for quantitative and qualitative observation of pollen grains through Light and scanning electron microscopy. Pollens of 12 species of Cyperaceae from different wetlands of Azad Jammu and Kashmir were collected. Morphological characters of pollen grains were then investigated under the Light and Scanning electron microscope. Two pollen types have been observed apolar and heteropolar. Shape of pollens was prolate (4 spp), sub-spheroidal (7 spp), and oblate (1 spp). Variation observed in exine sculpturing granular (4 spp), reticulate (1 spp), areolate-punctate (3 spp), and psilate (2 spp). Polar to equatorial ratio and fertility percentage of the pollens were also studied. Based on these micromorphlogical characters of pollens taxonomic keys have been made for the accurate identification of the members of Cyperaceae. The characteristics studied in present research work are very much valuable taxonomically and phytochemically for the identification of species of family Cyperaceae. Light microscope (LM) and Scanning electron microscope (SEM) were used for pollen observation, which play vital role in the taxonomical identification of species and provide sufficient information for taxonomist.

Keywords: Cyperaceae; LM; SEM; palynology; taxonomy.

MeSH terms

  • Cyperaceae / ultrastructure*
  • Microscopy, Electron, Scanning / methods*
  • Pollen / ultrastructure
  • Wetlands*