Anticancer Activity Study of Chromone and Coumarin Hybrids using Electrical Impedance Spectroscopy

Anticancer Agents Med Chem. 2018;18(6):854-864. doi: 10.2174/1871520618666180130102259.

Abstract

Aims: Oncology treatments aim at selective toxicity for tumor (compared to normal) cells, and chromone- coumarin hybrids have shown such activity.

Methods: In this study, we test a novel series of synthetic chromone and coumarin derivatives (1-9) for cytotoxic activity against a panel of tumor cell lines (MCF-7, A549, HepG2, HTC-116, B16 and Caco-2) opposed to non-tumor cells (HEK-293t). Electrical impedance spectroscopy was used to monitor cell viability in real time.

Results: Compound 8 showed the most potent activity, and it significantly diminished cancer cell proliferation and viability in different cell lines. It induced apoptosis in a dose-dependent manner, as shown by Western blot and flow cytometry.

Conclusion: Electrical impedance spectroscopy appears to be a convenient tool for in vitro cytotoxicity analysis, which could be useful for identifying drug effects and side effects during early phases of drug discovery and development.

Keywords: Coumarin; anticancer; cell sine; chromone; cytotoxicity; impedance..

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Chromones / chemistry
  • Chromones / pharmacology*
  • Coumarins / chemistry
  • Coumarins / pharmacology*
  • Dielectric Spectroscopy
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • Electric Impedance
  • Humans
  • Molecular Structure
  • Structure-Activity Relationship
  • Tumor Cells, Cultured

Substances

  • Antineoplastic Agents
  • Chromones
  • Coumarins
  • coumarin