Enzymatic Synthesis of GDP-α-l-fucofuranose by MtdL and Hyg20

Xiangjing Qin; Yunchang Xie; Hongbo Huang; Qi Chen; Junying Ma; Qinglian Li; Jianhua Ju

doi:10.1021/acs.orglett.7b03962

Enzymatic Synthesis of GDP-α-l-fucofuranose by MtdL and Hyg20

Org Lett. 2018 Feb 16;20(4):1015-1018. doi: 10.1021/acs.orglett.7b03962. Epub 2018 Jan 30.

Authors

Xiangjing Qin¹, Yunchang Xie¹, Hongbo Huang¹, Qi Chen¹, Junying Ma¹, Qinglian Li¹, Jianhua Ju^{1

2}

Affiliations

¹ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences , 164 West Xingang Road, Guangzhou 510301, China.
² University of Chinese Academy of Sciences , 19 Yuquan Road, Beijing 110039, China.

PMID: 29380608
DOI: 10.1021/acs.orglett.7b03962

Abstract

Two mutases, MtdL and Hyg20, are reported. Both are able to functionally drive the biosynthesis of GDP-α-l-fucofuranose. Both enzymes catalyze similar functions, catalytically enabling the bidirectional reaction between GDP-β-l-fucopyranose and GDP-α-l-fucofuranose using only divalent cations as cofactors. This realization is but one of a number of important insights into fucofuranose biosynthesis presented herein.

Publication types

Research Support, Non-U.S. Gov't