Objective: To understand the quality of blood smears for Plasmodium detection in Changzhou City, so as to provide the technical support for malaria surveillance in the post-elimination period.
Methods: The blood smears of fever patients from each cities (districts) in Changzhou City in 2014, including more than 3% of the negatives and all the positives, were selected. The quality of the blood smear making, dyeing, cleanliness and results were reviewed by the experts from the municipal malaria microscopy station, and the data were analyzed statistically.
Results: Totally 996 negative blood smears were reviewed in 2014, with a review rate of 4.52%. The qualified rates of blood smear making, dyeing and cleanliness were 92.87%, 93.27% and 94.48%, respectively. A total of 34 positive blood smears were reviewed, and there were no false detections and leak detections. The qualified rates of blood smear making and dyeing were more than 90% in all the seven cities (districts). As for qualified rate of cleanliness, except the rate in Qishuyan District (81.36%), those in the other six cities (districts) were all above 90%. The qualified rates of blood smear making, dyeing and cleanliness were all more than 90% in first-class, second-class and third-class hospitals. The major quality defects of the blood smears were sediment, abnormal making and thick membrane shedding, and the proportions were 25.91%, 21.76% and 19.17%, respectively.
Conclusions: The quality of blood smears for Plasmodium detection in Changzhou City is satisfying. In the future, the training and supervision still should be strengthened to guarantee the capacity of microscopic examinations for Plasmodium detection in the surveillance of post-elimination period.
［摘要］目的 了解常州市“三热”病人疟原虫检测血涂片制作质量, 为消除疟疾后的监测提供技术保障。方法 抽取 2014年常州市各市 (区) 不少于3%的已检“三热”病人疟原虫检测阴性血涂片和全部阳性血涂片, 市级疟疾镜检站对血涂 片的制作、染色、清洁度和镜检结果进行复核, 并对复核结果进行统计分析。结果 共复核阴性血涂片996张, 复核率为 4.52%, 血涂片制作、染色、清洁度合格率分别为92.87%、93.27%和94.48%; 复核阳性血涂片34张, 未发现误检和漏检。7 个市 (区) 血涂片制作、染色合格率均在90%以上; 除戚墅堰区清洁度合格率为81.36%外, 其他市 (区) 均＞90%。一级、二 级、三级医院血涂片制作、染色、清洁度合格率均＞90%。血涂片质量缺陷以沉渣、血膜制作不规范及厚膜脱落为主, 分 别占25.91%、21.76%和19.17%。结论 常州市各市 (区) 疟原虫血涂片质量较好, 今后要进一步加强培训和指导, 以保证 消除后监测阶段的疟原虫镜检能力。.
Keywords: Blood smear; Changzhou City; Fever patient; Malaria; Microscopy; Plasmodium; Quality.