Molecular characterization of a novel rhabdovirus infecting blackcurrant identified by high-throughput sequencing

Arch Virol. 2018 May;163(5):1363-1366. doi: 10.1007/s00705-018-3709-x. Epub 2018 Jan 24.

Abstract

A large contig with sequence similarities to several nucleorhabdoviruses was identified by high-throughput sequencing analysis from a black currant (Ribes nigrum L.) cultivar. The complete genome sequence of this new nucleorhabdovirus is 14,432 nucleotides long. Its genomic organization is very similar to those of unsegmented plant rhabdoviruses, containing six open reading frames in the order 3'-N-P-P3-M-G-L-5. The virus, which is provisionally named "black currant-associated rhabdovirus", is 41-52% identical in its genome nucleotide sequence to other nucleorhabdoviruses and may represent a new species in the genus Nucleorhabdovirus.

MeSH terms

  • Genome, Viral*
  • High-Throughput Nucleotide Sequencing
  • Open Reading Frames
  • Phylogeny
  • Plant Diseases / virology
  • RNA, Viral / genetics
  • Rhabdoviridae / classification
  • Rhabdoviridae / genetics*
  • Rhabdoviridae / isolation & purification
  • Rhabdoviridae / physiology
  • Rhabdoviridae Infections / virology*
  • Ribes / virology*

Substances

  • RNA, Viral