Alterations of oxygen consumption and extracellular acidification rates by glutamine in PBMCs of SLE patients

Hui-Ting Lee; Chen-Sung Lin; Siao-Cian Pan; Tsai-Hung Wu; Chyou-Shen Lee; Deh-Ming Chang; Chang-Youh Tsai; Yau-Huei Wei

doi:10.1016/j.mito.2018.01.002

Alterations of oxygen consumption and extracellular acidification rates by glutamine in PBMCs of SLE patients

Mitochondrion. 2019 Jan:44:65-74. doi: 10.1016/j.mito.2018.01.002. Epub 2018 Jan 11.

Authors

Hui-Ting Lee¹, Chen-Sung Lin², Siao-Cian Pan³, Tsai-Hung Wu⁴, Chyou-Shen Lee⁵, Deh-Ming Chang⁶, Chang-Youh Tsai⁷, Yau-Huei Wei⁸

Affiliations

¹ Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Medicine, Mackay Medical College, New Taipei City, Taiwan; Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, Taiwan.
² Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan; Division of Thoracic Surgery, Taipei Hospital, Ministry of Health and Welfare, New Taipei City, Taiwan.
³ Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan; Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, Taiwan; Center for Mitochondrial Medicine and Free Radical Research, Changhua Christian Hospital, Changhua City, Taiwan.
⁴ Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; Division of Nephrology, Taipei Veterans General Hospital, Taipei, Taiwan.
⁵ Mackay Junior College of Medicine, Nursing and Management, New Taipei City, Taiwan; Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, Taiwan.
⁶ Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; Division of Allergy, Immunology and Rheumatology, Taipei Veterans General Hospital, Taipei, Taiwan.
⁷ Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; Division of Allergy, Immunology and Rheumatology, Taipei Veterans General Hospital, Taipei, Taiwan. Electronic address: cytsai@vghtpe.gov.tw.
⁸ Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan; Department of Medicine, Mackay Medical College, New Taipei City, Taiwan; Center for Mitochondrial Medicine and Free Radical Research, Changhua Christian Hospital, Changhua City, Taiwan. Electronic address: 181765@cch.org.tw.

PMID: 29337141
DOI: 10.1016/j.mito.2018.01.002

Abstract

We evaluated plasma glutamine levels and basal mitochondrial oxygen consumption rate (mOCR_B) and basal extracellular acidification rate (ECAR_B) of peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematous (SLE) patients and healthy controls (HCs). Lower plasma glutamine levels correlated with higher SLE disease activity indexes (p=0.025). Incubated in DMEM containing 100mg/dL glucose, SLE-PBMCs displayed lower mOCR_B (p=0.018) but similar ECAR_B (p=0.467) to those of HC-PBMCs, and their mOCR_B got elevated (p<0.001) without altering ECAR_B (p=0.239) by supplementation with 2 or 4mM glutamine. We conclude that impaired mitochondrial respiration of SLE-PBMCs could be improved by glutamine under euglycemic condition.

Keywords: Extracellular acidification rate (ECAR); Glutamine; Oxygen consumption rate (OCR); Peripheral blood mononuclear cells (PBMCs); Systemic lupus erythematous (SLE).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Cohort Studies
Female
Glutamine / blood*
Humans
Leukocytes, Mononuclear / chemistry*
Leukocytes, Mononuclear / metabolism*
Lupus Erythematosus, Systemic / pathology*
Male
Middle Aged
Mitochondria / metabolism*
Oxygen Consumption*
Plasma / chemistry*

Substances

Glutamine