Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease

Ana Marote; Yuriy Pomeshchik; Stefano Goldwurm; Anna Collin; Nuno J Lamas; Luísa Pinto; António J Salgado; Laurent Roybon

doi:10.1016/j.scr.2018.01.007

Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease

Stem Cell Res. 2018 Mar:27:82-85. doi: 10.1016/j.scr.2018.01.007. Epub 2018 Jan 4.

Authors

Ana Marote¹, Yuriy Pomeshchik², Stefano Goldwurm³, Anna Collin⁴, Nuno J Lamas⁵, Luísa Pinto⁶, António J Salgado¹, Laurent Roybon⁷

Affiliations

¹ Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal; ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal.
² Stem Cell Laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, Lund, Sweden; Strategic Research Area MultiPark, Lund University, Lund, Sweden; Lund Stem Cell Center, Lund University, Lund, Sweden.
³ Parkinson Institute, ASST PINI-CTO, Milan, Italy.
⁴ Department of Clinical Genetics and Pathology, Office for Medical Services, Division of Laboratory Medicine, Lund, Sweden.
⁵ Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal; ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal; Anatomic Pathology Department, Braga Hospital, Braga, Portugal.
⁶ Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal; ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal; BnML, Behavioral and Molecular Lab, Braga, Portugal.
⁷ Stem Cell Laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, Lund, Sweden; Strategic Research Area MultiPark, Lund University, Lund, Sweden; Lund Stem Cell Center, Lund University, Lund, Sweden. Electronic address: laurent.roybon@med.lu.se.

PMID: 29334630
DOI: 10.1016/j.scr.2018.01.007

Abstract

An induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of common pluripotency markers, in vitro differentiation into three germ layers and normal karyotype. This iPSC line can be used to study the mechanisms underlying the development of PD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation / genetics
Cell Differentiation / physiology
Cell Line
Cellular Reprogramming / genetics
Cellular Reprogramming / physiology
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism*
Kruppel-Like Factor 4
Kruppel-Like Transcription Factors / genetics
Kruppel-Like Transcription Factors / metabolism
Male
Octamer Transcription Factor-3 / genetics
Octamer Transcription Factor-3 / metabolism
Parkinson Disease / metabolism*
Proto-Oncogene Proteins c-myc / genetics
Proto-Oncogene Proteins c-myc / metabolism
SOXB1 Transcription Factors / genetics
SOXB1 Transcription Factors / metabolism

Substances

KLF4 protein, human
Kruppel-Like Factor 4
Kruppel-Like Transcription Factors
MYC protein, human
Octamer Transcription Factor-3
POU5F1 protein, human
Proto-Oncogene Proteins c-myc
SOX2 protein, human
SOXB1 Transcription Factors