In Vivo Analysis of Calcium Levels and Glutathione Redox Status in Arabidopsis Epidermal Leaf Cells Infected with the Hypersensitive Response-Inducing Bacteria Pseudomonas syringae pv. tomato AvrB (PstAvrB)

Methods Mol Biol. 2018:1743:125-141. doi: 10.1007/978-1-4939-7668-3_12.

Abstract

Plants react to the attack of pathogen microorganisms by mounting appropriate and efficient downstream defense responses often involving a form of localized cell death called hypersensitive response (HR).Here we describe an innovative and noninvasive protocol based on in vivo bioimaging technique coupled with utilization of genetically encoded fluorescent sensors that allows to monitor and analyze intracellular calcium (Ca2+) dynamics and changes of the glutathione redox status taking place in plant organs during plant interaction with the HR-inducing bacteria Pseudomonas syringae (PstAvrB).

Keywords: Cameleon calcium sensor; GRX-roGFP2 sensor; Hypersensitive response; In vivo imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis / microbiology*
  • Calcium / metabolism*
  • Gene Expression
  • Genes, Reporter
  • Glutathione / metabolism*
  • Microscopy, Fluorescence
  • Molecular Imaging
  • Oxidation-Reduction*
  • Phenotype
  • Plant Leaves / metabolism*
  • Plant Leaves / microbiology*
  • Pseudomonas syringae / physiology*
  • Reactive Oxygen Species / metabolism
  • Reproducibility of Results

Substances

  • Reactive Oxygen Species
  • Glutathione
  • Calcium