Objective: To compare two influenza polymerase chain reaction (PCR) methods.
Methods: A total of 749 suspected MERS-CoV patients presenting at Johns Hopkins Aramco Healthcare, Saudi Arabia, each submitted a clinical sample for influenza A reflex testing using the on-site Cepheid® Xpert Flu assay and at the Ministry of Health laboratory by the Roche PCR assay.
Results: There was 92.12% overall agreement between the two methods. Specificity of the Cepheid® Xpert Flu was 95.8% for H1N1 and 94.4% for total influenza A. Cepheid® Xpert Flu sensitivity for influenza A was 100% for younger patients (0-19-year age group) but significantly lower both for older patients (68.2% for 60-79-year and 50% for ≥80-year age groups) and overall for males compared to females (72.6% and 94.0%, respectively).
Conclusions: Specificity of the Cepheid® Xpert Flu test was high; however, sensitivity for total influenza A was lower particularly in males and older patients.
Keywords: Cepheid® Xpert flu; Influenza A; Influenza A (H1N1) pdm09; Real-time PCR; Saudi Arabia.
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