Gene expression patterns associated with human placental trophoblast differentiation

Shi-Wen Jiang; Wei Zhou; Jianhao Wang; Lauren M Little; Lynn Leaphart; Jacob Jay; Eseosaserea Igbinigie; Haibin Chen; Jinping Li

doi:10.1016/j.cca.2018.01.012

Gene expression patterns associated with human placental trophoblast differentiation

Clin Chim Acta. 2019 Aug:495:637-645. doi: 10.1016/j.cca.2018.01.012. Epub 2018 Jan 10.

Authors

Shi-Wen Jiang¹, Wei Zhou², Jianhao Wang³, Lauren M Little⁴, Lynn Leaphart⁴, Jacob Jay⁵, Eseosaserea Igbinigie⁶, Haibin Chen⁷, Jinping Li⁸

Affiliations

¹ Department of Obstetrics and Gynecology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China; Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA; Department of Obstetrics and Gynecology, Memorial Health Hospital, Mercer University School of Medicine, Savannah, GA 31404, USA.
² Department of Obstetrics, Chongqing Health Center for Women and Children, Chongqing 400013, China.
³ School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu 213000, China.
⁴ Department of Obstetrics and Gynecology, Memorial Health Hospital, Mercer University School of Medicine, Savannah, GA 31404, USA.
⁵ Mercer University School of Medicine, Savannah, GA 31404, USA.
⁶ Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA.
⁷ Department of Histology & Embryology, Shantou University Medical College, Shantou, Guangdong 515041, China. Electronic address: chenhb@stu.edu.cn.
⁸ Department of Obstetrics and Gynecology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China; Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA; Department of Obstetrics and Gynecology, Memorial Health Hospital, Mercer University School of Medicine, Savannah, GA 31404, USA; School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu 213000, China. Electronic address: Li_J@mercer.edu.

PMID: 29329728
DOI: 10.1016/j.cca.2018.01.012

Abstract

Cell fusion is a hallmark of placental trophoblast cell differentiation and the mature syncytiotrophoblasts play essential roles for fetal-maternal exchange and production of pregnancy-related hormones. Using a well-established in vitro trophoblast differentiation model, we performed a microarray analysis on mRNA expression in trophoblast and syncytiotrophoblast cell cultures. Dramatic changes in gene expression patterns were detected during trophoblast differentiation. Real-time PCR analysis confirmed the reliability of the microarray data. As many as 3524 novel and known genes have been found to be up- or down-regulated for >2-fold. A number of cell cycle regulator including CDC6, CDC20, Cyclins B2, L1 and E2, were down-regulated in the syncytiotrophoblast, providing a mechanism for the loss of mitotic activity during trophoblast differentiation. Further characterization on the identified genes may lead to better understanding of placental patho-physiology in obstetric diseases such as preeclampsia.

Keywords: Cell fusion; Placental trophoblast; Syncytiotrophoblast; cDNA microarray.

MeSH terms

Cell Differentiation / genetics*
Female
Gene Expression Profiling*
Humans
Oligonucleotide Array Sequence Analysis
Pregnancy
Trophoblasts / cytology*
Trophoblasts / metabolism*