Novel amperometric bi-enzyme biosensor based on tyrosinase (Tyr)-catalyzed L-tyrosine polymerization to effectively immobilize Tyr and glucose oxidase (GOx) simultaneously has been developed and is demonstrated to be efficient in monitoring multi-analyte (bisphenol A (BPA), phenol, Cr(III), glucose, and Cr(VI)). For the first time, the polymer from L-tyrosine oxidation (PLT) was utilized to in-situ immobilize biomacromolecules and was demonstrated to be effective in entrapping high-load and high-activity enzymes. The prepared bi-enzyme biosensor (PLT-Tyr-GOx/Au electrode) works well in the biosensing of glucose (GOx substrate) and Cr(VI) (GOx inhibitor), and also exhibits excellent performance in Tyr substrates (BPA and phenol) and Tyr inhibitor (Cr(III)) sensing. In addition, the resultant biosensor exhibits excellent stability, precision, high sensitivity and fabrication simplicity, which may find wide applications in diversified fields including biotechnology.
Keywords: Bisphenol A; Cr(III); Cr(VI); Glucose biosensing; Oxidative polymerization of L-tyrosine; Phenol.
Copyright © 2017 Elsevier B.V. All rights reserved.