Plasma proteome analyses of the future promise invaluable insights into states of health, not only by measuring proteins whose role it is to ensure blood homeostasis, but increasingly also as a window into the health of practically any tissue in the body via so-called leakage protein biomarkers. Realizing more of this vast potential will require progress along many lines. Here we discuss the main ones, such as optimal selection of target proteins, affinity reagents, immunoassay formats, samples, and applications, with a view from ongoing work in our laboratory.
Keywords: Consecutive patient samples; Dried blood spots; Dual-recognition assays; Early detection of disease; Exocrine secretion; Liquid biopsy; Point of care; Protein leakage markers; Proximity extension assay; Proximity ligation assay; Recombinant protein binders; Sandwich immune assay; Tissue-specific proteins; Wellness testing.
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