The inhibitory effect of dinitrosyl iron complexes (NO donors) on myeloperoxidase activity

N P Akentieva; N A Sanina; A R Gizatullin; N Y Shmatko; N S Goryachev; N I Shkondina; T R Prikhodchenko; S M Aldoshin

doi:10.1134/S1607672917060126

The inhibitory effect of dinitrosyl iron complexes (NO donors) on myeloperoxidase activity

Dokl Biochem Biophys. 2017 Nov;477(1):389-393. doi: 10.1134/S1607672917060126. Epub 2018 Jan 3.

Authors

N P Akentieva¹, N A Sanina², A R Gizatullin², N Y Shmatko², N S Goryachev², N I Shkondina², T R Prikhodchenko², S M Aldoshin²

Affiliations

¹ Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow oblast, Russia. na_aken@icp.ac.ru.
² Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow oblast, Russia.

PMID: 29297116
DOI: 10.1134/S1607672917060126

Abstract

The effect of synthetic analogues of dinitrosyl mononuclear iron complexes (DNICs) with functional sulfur-containing ligands (NO donors) on the activity of myeloperoxidase (MPO) was studied, and their efficiency was evaluated. It was shown that the enzyme MPO is the molecular target of DNICs. It was found that six DNICs inhibited the activity of MPO and one compound potentiated it. The evaluation of their efficiency showed that two DNICs effectively inhibited the activity of MPO by 50% at IC₅₀ = 2 × 10^-4 M and IC₅₀ = 5 × 10^-7 M.

MeSH terms

Animals
Cell Adhesion / drug effects
Cells, Cultured
Enzyme Activation / drug effects
Iron / pharmacology*
Myocytes, Cardiac / drug effects*
Myocytes, Cardiac / enzymology
Nitrogen Oxides / pharmacology*
Peroxidase / antagonists & inhibitors*
Peroxidase / metabolism*
Rats

Substances

Nitrogen Oxides
dinitrosyl iron complex
Iron
Peroxidase