Objective: to determine the effectiveness of citrulline use for correcting endothelial dysfunction in children resi dents of radioactively contaminated areas.
Materials and methods: A group of children residents of radioactively contaminated areas with the presence of clinical and paraclinical signs of endothelial dysfunction was selected to assess the effectiveness of correcting endothelial dysfunction by the usage of NO potential donator - citrulline according to the data of selective screen ing. There were determined the biochemical parameters of the content of NO stable metabolites, L arginine, lipid peroxidation, antioxidant enzymes in the blood serum; the indices of cellular and humoral immunity; the instrumen tal indices of vascular endothelium dependent reaction on occlusion test, the lung ventilation capacity, the bioelec tric activity of the myocardium, the autonomic regulation of the cardiovascular system.Examined children were received a course of citrulline malate.
Results and conclusions: An increased content of serum L arginine, nitrite, and amounts of NO metabolites was established in children with endothelial dysfunction who were received a course of citrulline malate. Bronchospasm elimination was noted in the significant part of examined patients after the drug use. Decreased recovery period and increased period of hypercompensation for thermographic circulation index in the test with post occlusion reac tive hyperemia were detected by an evaluation of indicators for vascular endothelium dependent vasodilatation using thermographic method indicating an increased endothelial vasomotor capacity. There was tendency to improve the processes of autonomic regulation of the heart rhythm and repolarization of the heart muscle. The antioxidant effect of used citrulline malate course was determined as: decreased content of serum LPO end products that react with thiobarbituric acid under elevated activity of antioxidant - catalase. An increase in the percentage of T lymphocyte, normalization of their subpopulation composition was noted in dynamics of citrulline malate application.
Meta roboty: vyznachyty efektyvnist' zastosuvannia tsytrulinu dlia korektsiï endotelial'noï dysfunktsiï u ditey̆, meshkantsiv radioaktyvno zabrudnenykh terytoriy̆.Materialy ta metody. Dlia otsinky efektyvnosti korektsiï endotelial'noï dysfunktsiï iz zastosuvanniam po tentsiy̆nogo donatora NO tsytrulinu za danymy selektyvnogo skryningu bula vidibrana grupa ditey̆, meshkantsiv radioaktyvno zabrudnenykh terytoriy̆, z naiavnistiu klinichnykh ta paraklinichnykh oznak endotelial'noï dysfunktsiï. Vyznachalysia biokhimichni pokaznyky vmistu stabil'nykh metabolitiv NO, L argininu, perekysnogo okyslennia lipidiv, antyoksydantnykh fermentiv u syrovattsi krovi; pokaznyky klitynnogo ta gumoral'nogo imunitetu, instrumen tal'ni pokaznyky endoteliy̆zalezhnoï reaktsiï sudyn na okliuziy̆nu probu, ventyliatsiy̆noï spromozhnosti legeniv, bioelektrychnoï aktyvnosti miokardu, vegetatyvnoï reguliatsiï sertsevo sudynnoï systemy. Obstezheni dity otrymu valy kurs tsytrulinu malatu.Rezul'taty ta vysnovky. U ditey̆ z endotelial'noiu dysfunktsiieiu, iaki otrymuvaly kurs tsytrulinu malatu vsta novleno pidvyshchennia vmistu v syrovattsi krovi L argininu, nitrytu, sumy metabolitiv NO. Pislia zastosuvannia pre paratu sposterigalosia usunennia bronkhospazmu u znachnoï chastyny obstezhenykh. Otsinka pokaznykiv endoteliy̆za lezhnoï vazodylatatsiï sudyn za dopomogoiu termografichnogo metodu vyznachyla zmenshennia periodu vidnovlennia i zbil'shennia periodu giperkompensatsiï termografichnogo pokaznyka krovoobigu v probi z postokliuziy̆noiu reak tyvnoiu giperemiieiu, shcho svidchyt' pro pidvyshchennia vazomotornoï spromozhnosti endoteliiu. Vidmichalasia tendentsiia do polipshennia protsesiv vegetatyvnoï reguliatsiï sertsevogo rytmu i repoliaryzatsiï sertsevogo m’iaza. Vyznacheno an tyoksydantnyy̆ vplyv zastosovanogo kursu tsytrulinu malatu: znyzhennia vmistu u syrovattsi krovi kintsevykh pro duktiv perekysnogo okyslennia lipidiv, shcho reaguiut' z tiobarbiturovoiu kyslotoiu, pry pidvyshchenni aktyvnosti an tyoksydantu – katalazy. V dynamitsi zastosuvannia tsytrulinu malatu vidmichalos' pidvyshchennia vidsotka T limfo tsytiv, normalizatsiia ïkh subpopuliatsiy̆nogo skladu.
Keywords: children; citrulline; correction; endothelial dysfunction; radioactively contaminated territories.
Ye. I. Stepanova, I. Ye.Kolpakov, V. M. Zygalo, V. Yu. Vdovenko, V. H. Kondrashova, O. S. Leonovich, V. V. Kryzhanivska.