Human adipose derived stem cells regress fibrosis in a chronic renal fibrotic model induced by adenine

Juan José Rivera-Valdés; Jesus García-Bañuelos; Adriana Salazar-Montes; Leonel García-Benavides; Alfredo Dominguez-Rosales; Juan Armendáriz-Borunda; Ana Sandoval-Rodríguez

doi:10.1371/journal.pone.0187907

Human adipose derived stem cells regress fibrosis in a chronic renal fibrotic model induced by adenine

PLoS One. 2017 Dec 27;12(12):e0187907. doi: 10.1371/journal.pone.0187907. eCollection 2017.

Authors

Juan José Rivera-Valdés¹, Jesus García-Bañuelos¹, Adriana Salazar-Montes¹, Leonel García-Benavides², Alfredo Dominguez-Rosales³, Juan Armendáriz-Borunda^{1

4}, Ana Sandoval-Rodríguez¹

Affiliations

¹ Institute for Molecular Biology in Medicine and Gene Therapy, Department of Molecular Biology and Genomics, Health Sciences University Center, University of Guadalajara, Guadalajara, Jalisco, Mexico.
² Department of Biomedical Sciences, Tonala University Center, University of Guadalajara, Tonala, Jalisco, Mexico.
³ Chronic-Degenerative Diseases Institute, Health Sciences University Center, University of Guadalajara, Guadalajara, Jalisco, Mexico.
⁴ Tecnologico de Monterrey, Guadalajara, Jalisco, Mexico.

Abstract

Background and aims: ADSCs transplantation had been shown in some experimental models of kidney damage that it improves kidney function and reduces fibrosis. In this study we evaluated the effect of human adipose tissue-derived stem cell (hADSC) therapy in a chronic kidney damage experimental model.

Methods: A chronic kidney injury was induced by daily orogastric administration of adenine (100mg/kg) to male Wistar rats for 28 days. hADSCs were isolated, expanded and characterized before transplantation. hADSC administration was performed in a tail vein at a dose of 2 x106 cells/animal. Animals were sacrificed at 7 days post-treatment. The percentage of fibrotic tissue, serum and urine levels of urea, creatinine, total protein and renal mRNA of COL1A1, TGFB1, CTGF, ACTA2, IL6, IL10, TNF were analyzed.

Results: hADSCs treatment significantly reduces kidney fibrosis, improves urea and creatinine serum and urine levels, and diminishes COL1A1, TGFB1, CTGF, ACTA2 mRNA kidney levels.

Conclusions: These results showed that cell therapy using hADSCs improves renal function and reduces fibrosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenine / toxicity*
Adipose Tissue / cytology*
Animals
Cells, Cultured
Fibrosis / genetics
Gene Expression Profiling
Humans
Kidney Diseases / chemically induced
Kidney Diseases / genetics
Kidney Diseases / prevention & control*
Male
Rats
Rats, Wistar
Stem Cells / cytology*

Substances

Adenine

Grants and funding

JJRV received a scholarship from CONACYT to obtain his PhD as part of this project. The specific role of this author is articulated in the ‘author contributions’ section. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.