Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide: Design, Synthesis, and Application of a Lanthanide-Based Probe

Yao Yao; Loruhama Delgado-Rivera; Hamid Samareh Afsari; Liang Yin; Gregory R J Thatcher; Terry W Moore; Lawrence W Miller

doi:10.1021/acs.inorgchem.7b02533

Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide: Design, Synthesis, and Application of a Lanthanide-Based Probe

Inorg Chem. 2018 Jan 16;57(2):681-688. doi: 10.1021/acs.inorgchem.7b02533. Epub 2017 Dec 27.

Authors

Yao Yao¹, Loruhama Delgado-Rivera^{2

3}, Hamid Samareh Afsari¹, Liang Yin^{2

3}, Gregory R J Thatcher^{2

3}, Terry W Moore^{2

3}, Lawrence W Miller¹

Affiliations

¹ Department of Chemistry, University of Illinois at Chicago , 845 W. Taylor Street, MC 111, Chicago, Illinois 60607, United States.
² Department of Medicinal Chemistry and Pharmacognosy, UICentre for Drug Discovery, University of Illinois at Chicago , Chicago, Illinois 60612, United States.
³ University of Illinois Cancer Center, University of Illinois at Chicago , Chicago, Illinois 60607, United States.

Abstract

Hydrogen sulfide (H₂S) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for H₂S, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control millisecond-scale-lifetime-Tb(III)- or Eu(III)-emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively, and with high sensitivity to aqueous H₂S. The probe's potential for biological applications was verified by measuring the H₂S generated by the slow-releasing chemical-sulfide-donor GYY4147, by cystathionine γ-lyase (CSE), and by Na₂S-stimulated HeLa cells.

Grants and funding

R01 GM081030/GM/NIGMS NIH HHS/United States