Structural characterization and osteogenic bioactivity of a sulfated polysaccharide from pacific abalone (Haliotis discus hannai Ino)

Shuang Song; Bao Zhang; Sufeng Wu; Lu Huang; Chunqing Ai; Jinfeng Pan; Yi-Cheng Su; Zhongfu Wang; Chengrong Wen

doi:10.1016/j.carbpol.2017.11.022

Structural characterization and osteogenic bioactivity of a sulfated polysaccharide from pacific abalone (Haliotis discus hannai Ino)

Carbohydr Polym. 2018 Feb 15:182:207-214. doi: 10.1016/j.carbpol.2017.11.022. Epub 2017 Nov 6.

Authors

Shuang Song¹, Bao Zhang², Sufeng Wu², Lu Huang², Chunqing Ai³, Jinfeng Pan², Yi-Cheng Su⁴, Zhongfu Wang⁵, Chengrong Wen⁶

Affiliations

¹ School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, PR China. Electronic address: songs1008@163.com.
² School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China.
³ School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, PR China.
⁴ Seafood Research and Education Center, Oregon State University, Astoria, OR 97103, United States.
⁵ Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education and Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an 710069, PR China.
⁶ School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, PR China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, PR China. Electronic address: wencr2014@163.com.

PMID: 29279116
DOI: 10.1016/j.carbpol.2017.11.022

Abstract

Bone morphogenic protein-2 (BMP-2) is known to promote osteogenesis. To find novel adjuvants to enhance the activity of BMP-2, the present study investigated the structure BMP-2-induced osteogenic activity of a water-soluble polysaccharide from the gonad of pacific abalone (Haliotis discus hannai Ino) named AGSP. Through analysis of aldobiouronic acids released from AGSP, monosaccharide composition comparison of AGSP and its reduced product, and methylation analysis and NMR analysis of AGSP and its desulfated derivative, the main structure residue of AGSP was determined as →3)-GlcA(1→3)-Gal(1→ with sulfated branches comprised of prevelant Gal and minor Glc, and →4)-β-GlcA(1→2)-α-Man(1→ residue was also found. AGSP possessed a sulfate content of 12.4% with a relative molecular weight of 6.6kDa. AGSP strengthened alkaline phosphatase activity induced by BMP-2 in a dose dependent manner at 10-200μg/mL with 425% enhancement being observed at 200μg/mL, indicating AGSP could be an adjuvant candidate to enhance osteogenic activity of BMP-2.

Keywords: Abalone; Bone morphogenetic protein; Chondroitin sulfate; Chondroitin sulfate (PubChem CID: 24766); Heparin; Heparin (PubChem CID: 25244225); Polysaccharide.

MeSH terms

Animals
Bone Morphogenetic Protein 2 / chemistry
Bone Morphogenetic Protein 2 / metabolism
Cells, Cultured
Dose-Response Relationship, Drug
Gastropoda / chemistry*
Mice
Molecular Structure
Osteogenesis / drug effects*
Polysaccharides / chemistry
Polysaccharides / pharmacology*
Sulfates / chemistry
Sulfates / pharmacology*

Substances

BMP2 protein, human
Bone Morphogenetic Protein 2
Polysaccharides
Sulfates