Chemical Synthesis of Natural Polyubiquitin Chains through Auxiliary-Mediated Ligation of an Expressed Ubiquitin Isomer

Ling Xu; Jian-Feng Huang; Chen-Chen Chen; Qian Qu; Jing Shi; Man Pan; Yi-Ming Li

doi:10.1021/acs.orglett.7b03515

Chemical Synthesis of Natural Polyubiquitin Chains through Auxiliary-Mediated Ligation of an Expressed Ubiquitin Isomer

Org Lett. 2018 Jan 19;20(2):329-332. doi: 10.1021/acs.orglett.7b03515. Epub 2017 Dec 26.

Authors

Ling Xu^{1

2}, Jian-Feng Huang², Chen-Chen Chen², Qian Qu¹, Jing Shi², Man Pan³, Yi-Ming Li¹

Affiliations

¹ School of Biological and Medical Engineering, Hefei University of Technology , Hefei, Anhui 230009, China.
² Department of Chemistry, School of Life Science, University of Science and Technology of China , Hefei 230026, China .
³ Department of Biochemistry and Molecular Biology, University of Chicago , Chicago, Illinois 60637, United States.

PMID: 29278505
DOI: 10.1021/acs.orglett.7b03515

Abstract

An efficient method for the assembly of polyUb chains using auxiliary-modified Ub isomers is reported. This strategy takes advantages of auxiliary-mediated native chemical ligation between the distal Ub C-terminal hydrazide and the auxiliary of proximal Ub. Using removable protecting groups, Lys48-linked and Lys6-linked tri-Ub and even a mixed-linkage Lys6, Lys48-linked triUb in multimilligram quantities was made. These results demonstrate that this strategy yields natural polyubiquitin chains of desired length and linkage by using Ub isomer.

Publication types

Research Support, Non-U.S. Gov't