Structure and Molecular Dynamics Simulations of Protein Tyrosine Phosphatase Non-Receptor 12 Provide Insights into the Catalytic Mechanism of the Enzyme

Int J Mol Sci. 2017 Dec 26;19(1):60. doi: 10.3390/ijms19010060.

Abstract

Protein tyrosine phosphatase non-receptor 12 (PTPN12) is an important protein tyrosine phosphatase involved in regulating cell adhesion and migration as well as tumorigenesis. Here, we solved a crystal structure of the native PTPN12 catalytic domain with the catalytic cysteine (residue 231) in dual conformation (phosphorylated and unphosphorylated). Combined with molecular dynamics simulation data, we concluded that those two conformations represent different states of the protein which are realized during the dephosphorylation reaction. Together with docking and mutagenesis data, our results provide a molecular basis for understanding the catalytic mechanism of PTPN12 and its role in tumorigenesis.

Keywords: PTPN12; molecular dynamics; structure.

MeSH terms

  • Catalytic Domain
  • Crystallography, X-Ray
  • Humans
  • Molecular Dynamics Simulation
  • Mutation
  • Neoplasms / genetics
  • Neoplasms / metabolism
  • Protein Conformation
  • Protein Tyrosine Phosphatase, Non-Receptor Type 12 / chemistry*
  • Protein Tyrosine Phosphatase, Non-Receptor Type 12 / genetics
  • Protein Tyrosine Phosphatase, Non-Receptor Type 12 / metabolism
  • Substrate Specificity

Substances

  • PTPN12 protein, human
  • Protein Tyrosine Phosphatase, Non-Receptor Type 12