Abstract
It is now demonstrated that the sex-specific maternal-placental-fetal interaction plays an important role in placental functions and pathologies. Determination of fetal-sex may therefore be an important consideration in studies using placenta samples. In this present study, we describe a simple, fast, and cheap protocol, which allows the fetal-sex determination of placental tissues from various starting materials (villi or formalin-fixed, paraffin-embedded (FFPE) tissues, isolated cytotrophoblasts or cellular debris from whole cell lysates, and cDNA) by a single duplex PCR reaction followed by agarose gel electrophoresis.
Keywords:
Genomic DNA; PCR; Placenta; Sex determination; Trophoblast.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abortion, Induced
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Adult
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Cell-Free System / metabolism
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Cells, Cultured
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Cesarean Section
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Chorionic Villi / metabolism*
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DNA / chemistry
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DNA / isolation & purification
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DNA / metabolism*
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DNA, Complementary / chemistry
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DNA, Complementary / isolation & purification
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DNA, Complementary / metabolism
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Electrophoresis, Agar Gel
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Female
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Humans
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Kruppel-Like Transcription Factors / chemistry
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Kruppel-Like Transcription Factors / genetics
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Kruppel-Like Transcription Factors / metabolism*
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Male
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Placenta / cytology
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Placenta / metabolism*
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Pregnancy
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Pregnancy Trimester, First
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sex Determination Analysis*
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Term Birth
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Trophoblasts / cytology
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Trophoblasts / metabolism*
Substances
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DNA, Complementary
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Kruppel-Like Transcription Factors
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ZFY protein, human
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zinc finger protein, X-linked
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DNA