Fetal-sex determination of human placental tissues

Placenta. 2018 Jan:61:103-105. doi: 10.1016/j.placenta.2017.12.001. Epub 2017 Dec 5.

Abstract

It is now demonstrated that the sex-specific maternal-placental-fetal interaction plays an important role in placental functions and pathologies. Determination of fetal-sex may therefore be an important consideration in studies using placenta samples. In this present study, we describe a simple, fast, and cheap protocol, which allows the fetal-sex determination of placental tissues from various starting materials (villi or formalin-fixed, paraffin-embedded (FFPE) tissues, isolated cytotrophoblasts or cellular debris from whole cell lysates, and cDNA) by a single duplex PCR reaction followed by agarose gel electrophoresis.

Keywords: Genomic DNA; PCR; Placenta; Sex determination; Trophoblast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abortion, Induced
  • Adult
  • Cell-Free System / metabolism
  • Cells, Cultured
  • Cesarean Section
  • Chorionic Villi / metabolism*
  • DNA / chemistry
  • DNA / isolation & purification
  • DNA / metabolism*
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification
  • DNA, Complementary / metabolism
  • Electrophoresis, Agar Gel
  • Female
  • Humans
  • Kruppel-Like Transcription Factors / chemistry
  • Kruppel-Like Transcription Factors / genetics
  • Kruppel-Like Transcription Factors / metabolism*
  • Male
  • Placenta / cytology
  • Placenta / metabolism*
  • Pregnancy
  • Pregnancy Trimester, First
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sex Determination Analysis*
  • Term Birth
  • Trophoblasts / cytology
  • Trophoblasts / metabolism*

Substances

  • DNA, Complementary
  • Kruppel-Like Transcription Factors
  • ZFY protein, human
  • zinc finger protein, X-linked
  • DNA