[PrepFiler Express BTATM Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from Bone]

S C Ding; H C Zhang; L L Gao

doi:10.3969/j.issn.1004-5619.2017.05.014

[PrepFiler Express BTATM Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from Bone]

Fa Yi Xue Za Zhi. 2017 Oct;33(5):514-515. doi: 10.3969/j.issn.1004-5619.2017.05.014. Epub 2017 Oct 25.

[Article in Chinese]

Authors

S C Ding¹, H C Zhang¹, L L Gao¹

Affiliation

¹ Institute of Criminal Science and Technology, Hangzhou Public Security Bureau, Hangzhou 310016, China.

PMID: 29275558
DOI: 10.3969/j.issn.1004-5619.2017.05.014

Abstract

Objectives: To establish a convenient and rapid method for extracting DNA from bone.

Methods: Fifteen long bone samples were washed and sterilized. The skeletal fragments were obtained by electric drill, and lysed by PrepFiler Express BTA™ lysis buffer. DNA was then manually extracted by silicon microbeads for further analysis.

Results: STR genotyping was successfully obtained in 14 out of the 15 samples, and the detection rate was 93.33%.

Conclusions: The method for DNA extraction from bone established in present study is convenient, quick, effective, and with a strong applicability, which is worth spreading and applying.

Keywords: forensic genetics; DNA; PrepFiler Express BTA™; bone; silica-extraction method.

MeSH terms

Bone and Bones / chemistry*
DNA / isolation & purification*
DNA Fingerprinting*
Forensic Genetics* / methods
Genotype
Humans
Microsatellite Repeats / genetics
Microspheres*
Real-Time Polymerase Chain Reaction
Silicon

Substances

DNA
Silicon