To enrich the resource pool of endophytic fungi from plants which produce taxol, a taxol-producing endophytic fungus TMS-26 was isolated from the stem of Taxus Media. The result of high performance liquid chromatography (HPLC) showed that TMS-26 extract exhibited similar chromatographic peaks and retention time (4.545 min) with authentic taxol. Then mass spectrometry (MS) analysis further confirmed that TMS-26 extracts contained the same mass peaks with authentic taxol ((M+Na)+=876). These indicated that the isolated endophytic fungus TMS-26 can produce taxol. According to the morphological characteristics, the molecular analysis of 18S rDNA and internal transcribed spacer nuclear rDNA gene sequence, the fungus was identified as Aspergillus fumigatus TMS-26.
为丰富产紫杉醇植物内生真菌资源库，从曼地亚红豆杉Taxus media 茎中分离得到一株产紫杉醇的内生真菌TMS-26。通过对TMS-26 的发酵提取物进行高效液相色谱分析，发现其具有与紫杉醇标准品 (4.545 min)相近的色谱特征峰。进一步通过液质联用仪检测发现，内生真菌TMS-26 的发酵提取物中具有与紫杉醇标准品((M+Na)+=876) 相近的质谱特征峰，表明内生真菌TMS-26 能够产生紫杉醇。同时通过传统形态学分类鉴定方法和18S rDNA 序列分析、Internal-transcribed spacer (ITS) 序列分析等现代分子生物学分类鉴定方法，最终将内生真菌TMS-26 鉴定为曲霉属烟曲霉Aspergillus fumigatus，并命名为“烟曲霉TMS-26”。.
Keywords: 18S rDNA; Aspergillus fumigatus; ITS sequence; Taxus media; endophytic fungus; taxol.