Quantitative and Sensitive Detection of Chloramphenicol by Surface-Enhanced Raman Scattering

Yufeng Ding; Xin Zhang; Hongjun Yin; Qingyun Meng; Yongmei Zhao; Luo Liu; Zhenglong Wu; Haijun Xu

doi:10.3390/s17122962

Quantitative and Sensitive Detection of Chloramphenicol by Surface-Enhanced Raman Scattering

Sensors (Basel). 2017 Dec 20;17(12):2962. doi: 10.3390/s17122962.

Authors

Yufeng Ding^{1

2}, Xin Zhang^{3

4}, Hongjun Yin^{5

6}, Qingyun Meng^{7

8}, Yongmei Zhao⁹, Luo Liu¹⁰, Zhenglong Wu¹¹, Haijun Xu^{12

13}

Affiliations

¹ Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China. 2015200888@mail.buct.edu.cn.
² College of Science, Beijing University of Chemical Technology, Beijing 100029, China. 2015200888@mail.buct.edu.cn.
³ Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China. zhxin@mail.buct.edu.cn.
⁴ College of Science, Beijing University of Chemical Technology, Beijing 100029, China. zhxin@mail.buct.edu.cn.
⁵ Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China. yinhj@mail.buct.edu.cn.
⁶ College of Science, Beijing University of Chemical Technology, Beijing 100029, China. yinhj@mail.buct.edu.cn.
⁷ Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China. mengqy@mail.buct.edu.cn.
⁸ College of Science, Beijing University of Chemical Technology, Beijing 100029, China. mengqy@mail.buct.edu.cn.
⁹ Engineering Research Center for Semiconductor Integrated Technology, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China. ymzhao@semi.ac.cn.
¹⁰ Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China. liuluo@mail.buct.edu.cn.
¹¹ Analytical and Testing Center, Beijing Normal University, Beijing 100875, China. wuzl@bnu.edu.cn.
¹² Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China. hjxu@mail.buct.edu.cn.
¹³ College of Science, Beijing University of Chemical Technology, Beijing 100029, China. hjxu@mail.buct.edu.cn.

Abstract

We used surface-enhanced Raman scattering (SERS) for the quantitative and sensitive detection of chloramphenicol (CAP). Using 30 nm colloidal Au nanoparticles (NPs), a low detection limit for CAP of 10^-8 M was obtained. The characteristic Raman peak of CAP centered at 1344 cm^-1 was used for the rapid quantitative detection of CAP in three different types of CAP eye drops, and the accuracy of the measurement result was verified by high-performance liquid chromatography (HPLC). The experimental results reveal that the SERS technique based on colloidal Au NPs is accurate and sensitive, and can be used for the rapid detection of various antibiotics.

Keywords: Au nanoparticles; chloramphenicol; quantitative detection; surface-enhanced Raman scattering.