Platelet-derived growth factor BB stimulates differentiation of rat immature Leydig cells

J Mol Endocrinol. 2018 Jan;60(1):29-43. doi: 10.1530/JME-17-0222.

Abstract

Platelet-derived growth factor (PDGF) is one family of growth factors that regulate cell growth and differentiation. Rat Leydig cells express PDGF-β receptor (PDGFRB) during pubertal development. However, the mechanism of PDGF in the regulation of Leydig cell development is unclear. In the present study, rat immature Leydig cells were isolated from the testes of 35-day-old Sprague-Dawley rats and treated with 1 and 10 ng/mL of PDGF-BB. After 24 h of treatment, these cells were harvested for genomics profiling and the medium steroids were measured. 1 and 10 ng/mL PDGF-BB significantly increased androgen production by rat immature Leydig cells. Genomics profiling analysis showed that the expression levels of steroidogenic acute regulatory protein (Star) were increased by 2-fold. Further analysis showed that Fos expression level was increased 2- and 5-fold by 1 and 10 ng/mL PDGF-BB, respectively. In conclusion, PDGF-BB stimulated the differentiation of rat immature Leydig cells via regulating Star.

Keywords: Fos; Platelet-derived growth factor BB; rat immature Leydig cells; steroidogenic acute regulatory protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androgens / metabolism
  • Animals
  • Becaplermin
  • Biosynthetic Pathways / drug effects
  • Cell Differentiation / drug effects*
  • Cholesterol / biosynthesis
  • DNA / biosynthesis
  • Gene Expression Regulation / drug effects
  • Leydig Cells / cytology*
  • Leydig Cells / drug effects
  • Male
  • Oligonucleotide Array Sequence Analysis
  • Proto-Oncogene Proteins c-sis / pharmacology*
  • Rats, Sprague-Dawley
  • Real-Time Polymerase Chain Reaction
  • Reproducibility of Results

Substances

  • Androgens
  • Proto-Oncogene Proteins c-sis
  • Becaplermin
  • DNA
  • Cholesterol