The effects of chorionic gonadotropin (hCG) on the expression of the hTERT gene in combination with the conversion of the phenotype of naive T-cells and T-cells of immune memory in vitro were studied. hCG inhibited expression of hTERT mRNA in naive T-cells (CD45RA+) and immune memory T cells (CD45RO+), causing a decrease in the replicative potential of the cells. The presence of hCG in the culture led to the conversion of the phenotype of T-lymphocytes. hCG reduced the number of proliferating T-cells of immune memory, estimated by phenotypic signs by differential gating. hCG (10 IU/ml and 100 IU/ml) inhibited expression of CD25 by the studied populations, but did not modulate expression of the CD71 proliferation marker. Thus, hCG inhibited the functional activity of naive T-cells and T-cells of immune memory, which, in the context of pregnancy, can contribute to the formation of immune tolerance to the semi-allogenic fetus.
Izuchali éffekty khorionicheskogo gonadotropina (KhG) na ékspressiiu gena hTERT v sochetanii s konversieĭ fenotipa naivnykh T-kletok i T-kletok immunnoĭ pamiati v sisteme in vitro. Ustanovleno, chto KhG ugnetal ékspressiiu mRNK gena hTERT v naivnykh T-kletkakh (CD45RA+) i T-kletkakh immunnoĭ pamiati (CD45RO+), chto privodilo k snizheniiu replikativnogo potentsiala kletok. Prisutstvie KhG v kul'ture privodilo k konversii fenotipa T-limfotsitov. KhG snizhal chislo proliferiruiushchikh T-kletok immunnoĭ pamiati, otsenivaemykh po fenotipicheskim priznakam metodom differentsial'nogo geĭtirovaniia, podavlial ékspressiiu markera aktivatsii CD25 issleduemymi populiatsiiami, ne vliiaia na ékspressiiu CD71, assotsiirovannogo s proliferatsieĭ. Takim obrazom, KhG snizhal replikativnyĭ potentsial i podavlial aktivatsiiu T-kletok i T-kletok immunnoĭ pamiati, chto v kontekste beremennosti mozhet vnosit' vklad v formirovanie immunnoĭ tolerantnosti k poluallogennomu émbrionu.
Keywords: CD25; CD71; hTERT; human chorionic gonadotropin (HCG); memory T-cells; naive T cells; proliferation.