Transcriptomic responses of the freshwater snail (Parafossarulus striatulus) following dietary exposure to cyanobacteria

Fei Qiao; Kun Lei; Zicheng Li; Zhanliang Wei; Qing Liu; Libiao Yang; JianWu He; Lihui An; Hongli Qi; Song Cui

doi:10.1016/j.scitotenv.2017.12.112

Transcriptomic responses of the freshwater snail (Parafossarulus striatulus) following dietary exposure to cyanobacteria

Sci Total Environ. 2018 May 15:624:153-161. doi: 10.1016/j.scitotenv.2017.12.112. Epub 2017 Dec 14.

Authors

Fei Qiao¹, Kun Lei¹, Zicheng Li¹, Zhanliang Wei¹, Qing Liu¹, Libiao Yang¹, JianWu He², Lihui An³, Hongli Qi⁴, Song Cui⁵

Affiliations

¹ State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
² State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Tianjin key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China.
³ State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address: anlhui@163.com.
⁴ Tianjin key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China.
⁵ School of Water Conservancy and Civil Engineering, Northeast Agricultural University, HarBin 150030, China.

PMID: 29248704
DOI: 10.1016/j.scitotenv.2017.12.112

Abstract

Freshwater snails are promising bioindicators that can be used in ecotoxicological testing and ecological risk assessments. To screen molecular responses following mollusk exposure to algal blooms, whole transcriptome sequencing was performed with the freshwater snail (Parafossarulus striatulus) fed with blue algae (Microcystis aeruginosa). A total of 86,848 unigenes were assembled, and 10,413 unigenes were annotated in the TrEMBL, Pfam, KEGG, and SwissProt databases. In snails fed with both green and blue algae, a total of 276 differentially expressed unigenes were identified, though there were limited differences in snails fed with only green algae. In addition, ten randomly selected differentially expressed unigenes were analyzed in snails collected from Taihu Lake, China. The expression of four unigenes exhibited a trend consistent with that observed in transcriptome profiling of laboratory snails. The results of this study provide an invaluable resource for enhancing our understanding of ecotoxicology following the occurrence of algal blooms in lakes.

Keywords: Biomarker; Cyanobacteria; Eutrophication; Mollusks; Transcriptome.

MeSH terms

Animals
China
Cyanobacteria*
Dietary Exposure*
Environmental Monitoring
Eutrophication
Lakes
Sentinel Species / metabolism
Snails / metabolism*
Transcriptome*