Green synthesis of anisotropic zinc oxide nanoparticles with antibacterial and cytofriendly properties

M Saravanan; V Gopinath; Mukesh Kumar Chaurasia; Asad Syed; Fuad Ameen; N Purushothaman

doi:10.1016/j.micpath.2017.12.039

Green synthesis of anisotropic zinc oxide nanoparticles with antibacterial and cytofriendly properties

Microb Pathog. 2018 Feb:115:57-63. doi: 10.1016/j.micpath.2017.12.039. Epub 2017 Dec 14.

Authors

M Saravanan¹, V Gopinath², Mukesh Kumar Chaurasia³, Asad Syed⁴, Fuad Ameen⁴, N Purushothaman⁵

Affiliations

¹ Institute of Biomedical Sciences, College of Health Sciences, Mekelle University, Ethiopia; Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, 603203, India.
² Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia. Electronic address: yele.gopi@gmail.com.
³ Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, 603203, India.
⁴ Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.
⁵ Department of Genetic Engineering and SRM Research Institue, SRM University, Kattankulathur, 603203, India.

PMID: 29248514
DOI: 10.1016/j.micpath.2017.12.039

Abstract

Zinc oxide nanoparticles (ZnONPs) exhibit abundant biomedical applications. Anisotropic ZnONPs with a defined shape and size were synthesized using Bacillus megaterium (NCIM 2326) cell free extract as a bio-reductant. The study investigated the multidimensional effect of ZnONPs on Helicobacter pylori strains and assessed its biosafety in normal human mesenchymal stem cells (hMSc). The highly stable ZnONPs were produced using B. megaterium and Zinc nitrate as a precursor. The phase of ZnONPs formation and structural characterization were performed by UV- visible (UV-Vis), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and Field Emission Scanning electron microscopy (FESEM) analysis. Furthermore, the ZnONPs exhibited higher biocompatibility against human mesenchymal stem cells (hMSC) and proved to be potentially safe in mammalian cells. Corroborating the current investigation, we described the anti-H. Pylori dosage of ZnONPs was safe to hMSC and could efficiently use as nano-antibiotic.

Keywords: Antibacterial mechanism; Bacillus megaterium; Cytotoxicity; Helicobacter pylori; Nanoparticles.

MeSH terms

Anisotropy
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Apoptosis / drug effects
Bacillus megaterium / metabolism
Cell Membrane / drug effects
Green Chemistry Technology / methods*
Helicobacter pylori / drug effects
Humans
Materials Testing
Mesenchymal Stem Cells / drug effects
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Microbial Sensitivity Tests
Particle Size
Reactive Oxygen Species / metabolism
Zinc Oxide / metabolism
Zinc Oxide / pharmacology*

Substances

Anti-Bacterial Agents
Reactive Oxygen Species
Zinc Oxide