Hyperbaric oxygen treatment did not significantly affect radiation injury in the mandibular area of rats

Tonje Sønstevold; Anne Christine Johannessen; Rolf K Reed; Gerd S Salvesen; Linda Stuhr

doi:10.1016/j.oooo.2017.10.014

Hyperbaric oxygen treatment did not significantly affect radiation injury in the mandibular area of rats

Oral Surg Oral Med Oral Pathol Oral Radiol. 2018 Feb;125(2):112-119. doi: 10.1016/j.oooo.2017.10.014. Epub 2017 Nov 2.

Authors

Tonje Sønstevold¹, Anne Christine Johannessen², Rolf K Reed³, Gerd S Salvesen¹, Linda Stuhr⁴

Affiliations

¹ Department of Biomedicine, Faculty of Medicine and Dentistry, University of Bergen, Norway.
² The Gade Laboratory for Pathology, Department of Clinical Medicine, Faculty of Medicine and Dentistry, University of Bergen, Norway; Center for Cancer Biomarkers, University of Bergen, Norway.
³ Department of Biomedicine, Faculty of Medicine and Dentistry, University of Bergen, Norway; Center for Cancer Biomarkers, University of Bergen, Norway.
⁴ Department of Biomedicine, Faculty of Medicine and Dentistry, University of Bergen, Norway; Center for Cancer Biomarkers, University of Bergen, Norway. Electronic address: linda.stuhr@uib.no.

PMID: 29248424
DOI: 10.1016/j.oooo.2017.10.014

Abstract

Objective: Hyperbaric oxygen therapy (HBOT) has been used to enhance microcirculation and thereby oxygen tension in tissues. The present study aimed to investigate the effect of HBOT on radiation injury in the mandibular area of rats.

Study design: The left mandibles of rats were irradiated by external radiotherapy (15 Gy every other week for a total of 75 Gy). Four HBOT strategies were used: 2 prophylactic groups receiving HBOT either between each radiation treatment or immediately following terminated radiation treatment, and 2 therapeutic groups receiving HBOT after the latent period of 6 weeks after irradiation either every day (standard HBOT protocol) or 3 days a week for 6 weeks. Tissue samples of the irradiated area were taken from skin, the salivary gland, and the mandible. All tissues were stained with hematoxylin and eosin for morphologic examination. Furthermore, skin samples were stained with CD31 for blood vessel analysis.

Results: There was no change in blood vessel density or morphology between controls and HBOT tissues after radiation. The dentin of 2 of the 5 rats that received HBOT either normalized or was not affected by irradiation.

Conclusions: HBOT did not affect radiation injury of the mandibular area in rats within 12 weeks after irradiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Hyperbaric Oxygenation / methods*
Male
Mandible / blood supply
Mandible / radiation effects*
Microcirculation
Radiation Dosage
Radiation Injuries / therapy*
Random Allocation
Rats
Rats, Sprague-Dawley