Pigmentation on gingiva can be physiologic or pathologic. Gingival pigmentation is present from birth in certain individuals. However, sudden occurrence of pigmentation suggests some systemic association. In the present case, a patient was referred by a physician for biopsy of the tissue along with gingival depigmentation. The depigmentation procedure was performed in both arches, and the biopsy specimen was sent for histopathologic examination. This case highlights the importance of biopsy of gingival pigmentation with a history of sudden occurrence. A biopsy is warranted to distinguish pathologic pigmentation from physiologic.