Abstract
The far-red emissive fluorescent probe CaPF-1 based on a phospha-fluorescein scaffold enables the detection of cytosolic calcium ions in living cells. The probe can be excited in the red region (λabs = 636 nm) and exhibits a sufficiently high fluorescence turn-on response in the far-red region (λem = 663 nm) upon complexation with calcium ions. The hydrophilic and anionic characteristics of this phospha-fluorescein fluorophore allowed the cytosolic localization of CaPF-1. Moreover, it was possible to visualize histamine-induced calcium oscillation in HeLa cells using CaPF-1.
MeSH terms
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Calcium / analysis*
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Calcium / metabolism
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Cyclic P-Oxides / chemical synthesis
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Cyclic P-Oxides / chemistry
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Cyclic P-Oxides / pharmacology*
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Fluoresceins / chemical synthesis
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Fluoresceins / chemistry
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Fluoresceins / pharmacology*
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Fluorescence
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / pharmacology*
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HeLa Cells
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Histamine / metabolism
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Humans
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Hydrogen-Ion Concentration
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Microscopy, Confocal
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Molecular Imaging
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Optical Imaging
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Receptors, Histamine H1 / metabolism
Substances
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Cyclic P-Oxides
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Fluoresceins
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Fluorescent Dyes
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Receptors, Histamine H1
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Histamine
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Calcium