Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxyphenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) against ischemic and hypoxic neuronal injury

Chang-Tsen Hung; Li-Dian Chen; Chien-Wei Hou

doi:10.22038/IJBMS.2017.9543

Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxyphenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) against ischemic and hypoxic neuronal injury

Iran J Basic Med Sci. 2017 Dec;20(12):1324-1330. doi: 10.22038/IJBMS.2017.9543.

Authors

Chang-Tsen Hung¹, Li-Dian Chen², Chien-Wei Hou³

Affiliations

¹ Department of Health and Leisure Management Yuanpei University of Medical Technology, Hsinchu, Taiwan.
² Department of Convalescence Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou China.
³ Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, Hsinchu, Taiwan.

Abstract

Objectives: Stroke may cause severe neuronal damage. The sesamin have been demonstrated to possess neuroprotection by its antioxidant and anti-inflammatory properties. One sesamin derivative was artificially composited, 1, 2-bis [(3-methoxyphenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) had been developed to study its antioxidative activity and neuroprotection.

Materials and methods: The infaction of Sprague Dawley (SD) rats and hypoxia models of BV-2 microglia or PC12 cells were investigated for in vivo and in vitro test respectively. Lipid peroxidation and reactive oxygen species (ROS), prostaglandin E₂ (PGE₂) and related signaling pathways from hypoxic cells were analyzed by ELISA or Western blot assay, respectively.

Results: MMEDA showed a protective effect when given 90 min after the focal cerebral ischemia. The neuroprotection of MMEDA was further confirmed by attenuating ROS and PGE₂ release from hypoxic BV-2 or PC12 cells. MMEDA significantly reduced hypoxia-induced JNK and caspase-3 (survival and apoptotic pathways) in PC12 cells.

Conclusion: The neuroprotective effect of MMEDA on ischemia/hypoxia models was involved with its antioxidative activity and anti-inflammatory effects. These results suggest that MMEDA exert effective neuroprotection against ischemia/hypoxia injury.

Keywords: Anti-inflammatory; Cerebral ischemia; Hypoxia; Neuroprotection; Reactive oxygen species Sesamin derivative.