Structural insight into catalytic mechanism of PET hydrolase

Nat Commun. 2017 Dec 13;8(1):2106. doi: 10.1038/s41467-017-02255-z.

Abstract

PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Binding Sites / genetics
  • Biocatalysis
  • Burkholderiales / enzymology*
  • Burkholderiales / genetics
  • Burkholderiales / metabolism
  • Catalytic Domain
  • Hydrolases / chemistry
  • Hydrolases / genetics
  • Hydrolases / metabolism*
  • Models, Molecular
  • Molecular Structure
  • Mutagenesis, Site-Directed
  • Polyethylene Terephthalates / chemistry
  • Polyethylene Terephthalates / metabolism*
  • Protein Conformation
  • Substrate Specificity

Substances

  • Bacterial Proteins
  • Polyethylene Terephthalates
  • Hydrolases