Abstract
PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Binding Sites / genetics
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Biocatalysis
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Burkholderiales / enzymology*
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Burkholderiales / genetics
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Burkholderiales / metabolism
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Catalytic Domain
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Hydrolases / chemistry
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Hydrolases / genetics
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Hydrolases / metabolism*
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Models, Molecular
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Molecular Structure
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Mutagenesis, Site-Directed
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Polyethylene Terephthalates / chemistry
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Polyethylene Terephthalates / metabolism*
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Protein Conformation
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Substrate Specificity
Substances
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Bacterial Proteins
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Polyethylene Terephthalates
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Hydrolases