Cohort-friendly protocol for a sensitive and fast method for trihalomethanes in urine using gas chromatography-Triple quadrupole mass spectrometry

Pantelis Charisiadis; Konstantinos C Makris

doi:10.1016/j.jchromb.2017.11.045

Cohort-friendly protocol for a sensitive and fast method for trihalomethanes in urine using gas chromatography-Triple quadrupole mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 1:1072:336-340. doi: 10.1016/j.jchromb.2017.11.045.

Authors

Pantelis Charisiadis¹, Konstantinos C Makris²

Affiliations

¹ Cyprus International Institute for Environmental and Public Health, Cyprus University of Technology, Limassol, Cyprus.
² Cyprus International Institute for Environmental and Public Health, Cyprus University of Technology, Limassol, Cyprus. Electronic address: konstantinos.makris@cut.ac.cy.

PMID: 29223046
DOI: 10.1016/j.jchromb.2017.11.045

Abstract

A cohort-friendly biomonitoring protocol has been developed for measuring biomarkers of exposure to disinfection by-products (trihalomethanes, THM) in urine using small initial volume (3mL) and short analysis time (∼10min) that facilitates the throughput of a large number of samples. The objective of this study was to optimise a cohort-friendly biomonitoring protocol for the determination of four THM analytes in human urine using gas chromatography coupled with triple quadrupole mass spectrometry (GC-QqQ-MS/MS). The proposed methodology will facilitate the inclusion of such urinary THM measurements into large population health studies.

Keywords: Biobanks; Biomarkers; Chlorine; Cohorts; Disinfection by products; Exposure.

MeSH terms

1-Propanol
Acetone
Gas Chromatography-Mass Spectrometry / methods*
Humans
Limit of Detection
Linear Models
Reproducibility of Results
Tandem Mass Spectrometry / methods*
Trihalomethanes / chemistry
Trihalomethanes / isolation & purification
Trihalomethanes / urine*

Substances

Trihalomethanes
Acetone
1-Propanol