In the enteric nervous system, neurons make contact with smooth muscle cells to regulate gastrointestinal functions. Since neuronal cell alterations and intestinal motor dysfunctions are strictly linked, in vitro models based on the culture of neurons or smooth muscle cells are of great advantage to elucidate the functions of the enteric nervous system under physiological and pathological conditions. In this chapter, we provide the protocol for isolation of neurons and smooth muscle cells from the myenteric plexus of adult mice. The isolated cells are suitable for immunocytochemical applications or electrophysiological investigations and/or co-culturing experiments.
Keywords: Enteric nervous system; Enzymatic digestion; Ganglia; Glial cell line-derived neurotropic factor; Longitudinal muscle/myenteric plexus.