Frequency-Dependent Multi-Well Cardiotoxicity Screening Enabled by Optogenetic Stimulation

Int J Mol Sci. 2017 Dec 6;18(12):2634. doi: 10.3390/ijms18122634.

Abstract

Side effects on cardiac ion channels causing lethal arrhythmias are one major reason for drug withdrawals from the market. Field potential (FP) recording from cardiomyocytes, is a well-suited tool to assess such cardiotoxic effects of drug candidates in preclinical drug development, but it is currently limited to the spontaneous beating of the cardiomyocytes and manual analysis. Herein, we present a novel optogenetic cardiotoxicity screening system suited for the parallel automated frequency-dependent analysis of drug effects on FP recorded from human-induced pluripotent stem cell-derived cardiomyocytes. For the expression of the light-sensitive cation channel Channelrhodopsin-2, we optimised protocols using virus transduction or transient mRNA transfection. Optical stimulation was performed with a new light-emitting diode lid for a 96-well FP recording system. This enabled reliable pacing at physiologically relevant heart rates and robust recording of FP. Thereby we detected rate-dependent effects of drugs on Na⁺, Ca2+ and K⁺ channel function indicated by FP prolongation, FP shortening and the slowing of the FP downstroke component, as well as generation of afterdepolarisations. Taken together, we present a scalable approach for preclinical frequency-dependent screening of drug effects on cardiac electrophysiology. Importantly, we show that the recording and analysis can be fully automated and the technology is readily available using commercial products.

Keywords: cardiomyocytes; cardiotoxicity screening; field potential; heart rate; induced pluripotent stem cells; long QT syndrome; optogenetics.

MeSH terms

  • Action Potentials
  • Cardiotoxicity
  • Cell Line
  • High-Throughput Screening Assays / instrumentation
  • High-Throughput Screening Assays / methods*
  • Humans
  • Induced Pluripotent Stem Cells / drug effects
  • Induced Pluripotent Stem Cells / physiology
  • Ion Channels / metabolism
  • Membrane Transport Modulators / toxicity*
  • Myocytes, Cardiac / drug effects*
  • Myocytes, Cardiac / physiology
  • Optogenetics / instrumentation
  • Optogenetics / methods*
  • Toxicity Tests / instrumentation
  • Toxicity Tests / methods*

Substances

  • Ion Channels
  • Membrane Transport Modulators