Protein folding and assembly can be manipulated in in vitro systems by co-solvents at high concentrations. A number of co-solvents that enhance protein stability and assembly have been shown to be excluded from the protein surface. Such co-solvent exclusion has been demonstrated by dialysis experiments and shown to be correlated with their effects on protein stability and assembly.
Keywords: Co-solvents; Dialysis equilibrium; In vitro systems; Preferentially excluded co-solvents; Protein folding and assembly.