Background: Type 2 diabetes mellitus (T2DM) is a huge burden in China. The Chinese patients with T2DM have several special clinical characteristics. Metabolomics studies predominantly have identified several distinguishing metabolites associated with T2DM in Western ancestry population. However, few previous metabolomics studies were conducted in Chinese populations.
Methods: We performed untargeted serum metabolic profiling between 30 T2DM patients and 30 healthy controls based on GC/TOFMS. Multivariate data analyses were applied to identify the distinguishing metabolites.
Results: Excellent separation was obtained between the two cases. And overall 54 distinguishing metabolites were identified with VIP>1 and P<0.05, which were involved in metabolic pathways of amino acid, carbohydrate, lipids, membrane transport and nucleotides. To further analyze the correlation between the identified metabolites and T2DM, 17 metabolites were selected with FC>2.0, including gentisic acid, citraconic acid, succinic acid, 2-hydroxybutanoic acid and 3-hydroxy-l-proline, the corresponding FC were respectively 5.44, 2.21, 2.10, 2.21 and -2.04.
Conclusion: Our results demonstrated that untargeted GC/TOFMS-based metabolic approach processed well performance to identify serum distinguishing metabolites of T2DM in Chinese adults, which may be as potential biomarkers in diagnose and treatment of diabetes. And the results also provided new insight into understand the underlying molecular mechanism.
Keywords: Chinese; GC/TOFMS; Metabolic profiling; Serum; T2DM.
Copyright © 2017. Published by Elsevier B.V.