Spatial (cap & stipe) metabolomic variations affect functional components between brown and white beech mushrooms

Food Res Int. 2017 Dec:102:544-552. doi: 10.1016/j.foodres.2017.09.043. Epub 2017 Sep 19.

Abstract

The beech mushrooms have customarily been revered by oriental societies for their nutritional and health benefits. We explored the mass spectrometry (MS) based spatial metabolomic variations between parts (cap and stipe) of two beech mushroom strains (brown and white). The principal component analysis (PCA) revealed their distinct primary (cap and stipe: PC1, 25.5%; strains: PC2, 12.5%) and secondary (cap and stipe: PC1, 10.3%; strains: PC2, 7.6%) metabolite patterns. The caps were rich in amino acids, fatty acids, and N-acetylglucosamine with higher protein and nitrogen contents. The stipes had abundant β-glucans, malic acid, and fructose. The discriminant secondary metabolites, especially, hypsiziprenols were higher in caps from brown strains. A fatty acid derivative, azelaic acid, was abundant in white strains (cap>stipe). We established a positive correlation for the cytotoxic activities of hypsiziprenols against ACHN cells. These spatial inter-strain metabolomic distinctions are potentially helpful for mushroom selection and improvement.

Keywords: Beech mushrooms; Cytotoxic activity; GC-TOF-MS; Hypsiziprenol; Metabolite profiling; UHPLC-MS/MS.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosamine / analysis
  • Agaricales / chemistry*
  • Amino Acids / analysis
  • Fatty Acids / analysis
  • Fructose / analysis
  • Fruiting Bodies, Fungal / chemistry
  • Malates
  • Mass Spectrometry / methods
  • Metabolomics*
  • Nitrogen / analysis
  • Principal Component Analysis
  • Proteins / analysis
  • Species Specificity
  • beta-Glucans / analysis

Substances

  • Amino Acids
  • Fatty Acids
  • Malates
  • Proteins
  • beta-Glucans
  • Fructose
  • malic acid
  • Nitrogen
  • Acetylglucosamine