Atmospheric CO2 enrichment effect on the Cu-tolerance of the C4 cordgrass Spartina densiflora

Abstract

A glasshouse experiment was designed to investigate the effect of the co-occurrence of 400 and 700ppm CO₂ at 0, 15 and 45mM Cu on the Cu-tolerance of C₄ cordgrass species Spartina densiflora, by measuring growth, gas exchange, efficiency of PSII, pigments profiles, antioxidative enzyme activities and nutritional balance. Our results revealed that the rising atmospheric CO₂ mitigated growth reduction imposed by Cu in plants grown at 45mM Cu, leading to leaf Cu concentration bellow than 270mgKg^-1 Cu, caused by an evident dilution effect. On the other hand, non-CO₂ enrichment plants showed leaf Cu concentration values up to 737.5mgKg^-1 Cu. Furthermore, improved growth was associated with higher net photosynthetic rate (A_N). The beneficial effect of rising CO₂ on photosynthetic apparatus seems to be associated with a reduction of stomatal limitation imposed by Cu excess, which allowed these plants to maintain greater _iWUE values. Also, plants grown at 45mM Cu and 700ppm CO₂, showed higher ETR values and lower energy dissipation, which could be linked with an induction of Rubisco carboxylation and supported by the recorded amelioration of N imbalance. Furthermore, higher ETR values under CO₂ enrichment could lead to an additional consumption of reducing equivalents. Idea that was reflected in the lower values of ETR_max/A_N ratio, malondialdehyde (MDA) and ascorbate peroxidase (APx), guaiacol peroxidase (GPx) and superoxide dismutase (SOD) activities under Cu excess, which could indicate a lower production of ROS species under elevated CO₂ concentration, due to a better use of absorbed energy.

Keywords: CO(2) enrichment; Chlorophyll fluorescence; Cu-stress; Gas exchange; Growth; Spartina densiflora.