A new antimicrobial bilayer film was developed using chitosan, cellulose, and nisin. Chitosan solution containing nisin (500 and 1000μg/mL) was prepared by sol-gel method and then the solution was coated on cellulose paper by dip coating method. A chitosan-cellulose film without antimicrobial had no inhibitory effect on Listeria monocytogenes, whereas, the incorporation of nisin made a significant increase (P<0.05) in antimicrobial characteristics of the films. Moreover, no significant differences were shown on antimicrobial activity of developed films during the storage at 4°C for one month. However, the addition of nisin showed a significant increase in the swelling index and solubility of bilayer film. Scanning electron microscope images revealed a uniform coating of chitosan solution on cellulose paper. The FTIR analysis also confırmed successful introducing and binding of the nisin in double layer film. Films with 1000μg/mL of nisin completely inactivated the initial (∼5log10 CFU/g) counts of L. monocytogenes on the surface of Ultra-filter white cheese after storage at 4°C for 14 days. We concluded that nanocomposite film of chitosan-cellulose containing nisin has novel antibacterial activity and can be used for packaging in cheese.
Keywords: Active packaging; Antibacterial; Chitosan-zinc oxide; Nanocomposite; Sol-gel; UF cheese.
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