Bioluminescence probe for γ-glutamyl transpeptidase detection in vivo

Bioorg Med Chem. 2018 Jan 1;26(1):134-140. doi: 10.1016/j.bmc.2017.11.025. Epub 2017 Nov 14.

Abstract

To detect γ-Glutamyl Transpeptidase (GGT) activity in vitro and in vivo, a bioluminescence probe with high sensitivity and specificity was well designed and synthesized. This probe can be recognized by GGT and release strong bioluminescence with its further reaction with luciferase. The performance of this probe was demonstrated in vitro and in cells. Finally, we applied the probe for detection of GGT activity in xenograft model.

Keywords: Bioluminescence probe; In vivo imaging; γ-Glutamyl transpeptidase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Female
  • Humans
  • Luminescent Agents / administration & dosage
  • Luminescent Agents / chemistry*
  • Luminescent Agents / pharmacology
  • Luminescent Measurements
  • Mice
  • Mice, Nude
  • Molecular Structure
  • Neoplasms, Experimental / drug therapy
  • Neoplasms, Experimental / metabolism
  • Neoplasms, Experimental / pathology
  • Ovarian Neoplasms / drug therapy
  • Ovarian Neoplasms / metabolism*
  • Ovarian Neoplasms / pathology
  • Structure-Activity Relationship
  • gamma-Glutamyltransferase / analysis*
  • gamma-Glutamyltransferase / metabolism

Substances

  • Luminescent Agents
  • gamma-Glutamyltransferase