Type 2 diabetes mellitus, a disease which prevalence has been progressively increasing worldwide, is characterized by chronic hyperglycemia resulting from the combination of inappropriate insulin secretion and/or resistance to insulin action. If left uncontrolled, diabetes is associated with complications such as dysfunction and failure of various organs, and even premature death. Along with lifestyle-modification strategies, several classes of oral antidiabetic agents can be employed for glycemic control. Thus, therapeutic drug monitoring of these drugs is essential to maintain appropriate treatment. This review discusses the most frequently employed analytical techniques and sample preparation systems to obtain a reliable and trustworthy method to quantify antidiabetic drugs in biological matrices. An adequate choice of internal standard, ideal chromatography conditions and most suitable analytical detector are reported.
Keywords: analytical techniques; antidiabetic agents; bioanalytical methods; diabetes; sample preparation.