Compact in-line lensfree digital holographic microscope

Manon Rostykus; Ferréol Soulez; Michael Unser; Christophe Moser

doi:10.1016/j.ymeth.2017.11.008

Compact in-line lensfree digital holographic microscope

Methods. 2018 Mar 1:136:17-23. doi: 10.1016/j.ymeth.2017.11.008. Epub 2017 Nov 21.

Authors

Manon Rostykus¹, Ferréol Soulez², Michael Unser³, Christophe Moser⁴

Affiliations

¹ Laboratory of Applied Photonics Devices, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. Electronic address: manon.rostykus@epfl.ch.
² Biomedical Imaging Group, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland; Univ Lyon, Univ Lyon1, Ens de Lyon, CNRS, Centre de Recherche Astrophysique de Lyon UMR5574, F-69230 Saint-Genis-Laval, France.
³ Biomedical Imaging Group, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
⁴ Laboratory of Applied Photonics Devices, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Abstract

Phase imaging provides intensity contrast to visualize transparent samples such as found in biology without any staining. Among them, digital holographic microscopy (DHM) is a well-known quantitative phase method. Lensfree implementations of DHMs offer the added advantage to provide large field of views (several mm² compared to several hundred μm²) and more compact setups that traditional DHM which have high quality microscope objectives. In this article, a lensfree DHM is presented using a side illumination technique in order to further reduce the device size. Its practical use is described and results on a transparent (phase only) sample are shown.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Holography / methods*
Humans
Image Processing, Computer-Assisted
Lenses*
Microscopy / methods*