Derivation and characterization of the NYSCFe003-A human embryonic stem cell line

Ana Sevilla; Eliana Forero; Matthew Zimmer; Hector Martinez; Katie Reggio; Daniel Paull; Dieter Egli; Scott Noggle

doi:10.1016/j.scr.2017.10.017

Derivation and characterization of the NYSCFe003-A human embryonic stem cell line

Stem Cell Res. 2017 Dec:25:217-220. doi: 10.1016/j.scr.2017.10.017. Epub 2017 Nov 21.

Authors

Ana Sevilla¹, Eliana Forero², Matthew Zimmer², Hector Martinez², Katie Reggio², Daniel Paull², Dieter Egli², Scott Noggle²

Affiliations

¹ The New York Stem Cell Foundation Research Institute, NY 10019, New York, United States. Electronic address: asevilla@nyscf.org.
² The New York Stem Cell Foundation Research Institute, NY 10019, New York, United States.

PMID: 29161648
DOI: 10.1016/j.scr.2017.10.017

Abstract

The human embryonic stem cell line NYSCFe003-A was derived from a day 5 to day 6 blastocyst in feeder-free and antibiotic free conditions. The blastocyst was voluntarily donated for research as surplus after in vitro fertilization treatment following informed consent. The NYSCFe003-A line expresses all the pluripotency markers and has the potential to differentiate into all three germ layers in vitro. The line presents normal karyotype and is mycoplasma free.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blastocyst / cytology
Cell Line
Cells, Cultured
Genotype
Human Embryonic Stem Cells / cytology*
Human Embryonic Stem Cells / metabolism
Humans
Karyotype
Male
Microscopy, Fluorescence